Fig. 2: Identification of genes required for PMD response in budding yeast; PMD limits replicative lifespan in budding yeast.
a, The genes required for the growth in the presence of 0.02% SDS. b, ESCRT mutants were spotted on YPD plates (4× dilution series) with or without 0.02% SDS, incubated at 25 °C for 3 d. c, Enriched GO terms. See also Supplementary Table 1. d, Fold enrichment scores in the GO enrichment analysis were plotted. e, Wild-type, snf7Δ, vps34Δ and erg2Δ were subjected to the replicative lifespan measurement. Median values are shown. Cell number: wild-type (n = 24), vps34Δ (n = 24), snf7Δ (n = 24) and erg2Δ (n = 25). f, Wild-type, VPS4-overexpressing or SNF7-overexpressing yeast cells were subjected to the replicative lifespan measurement. Median values are shown. Cell number: wild-type (n = 48), VPS4 (n = 48), SNF7 (n = 48). g, Wild-type, VPS4-overexpressing or SNF7-overexpressing yeast cells were subjected to the replicative lifespan measurement with or without 0.02% SDS. Median values are shown. Cell number: wild-type (n = 24), SDS (n = 24), SDS+VPS4 (n = 24) and SDS+SNF7 (n = 25). *P < 0.05, **P < 0.01 and ****P < 0.001, by two-sided Wilcoxon rank-sum test. Exact P value: wild-type versus vps34Δ: <0.0001, wild-type versus snf7Δ: <0.0001 and wild-type versus erg2Δ: 0.0029 (e); wild-type versus VPS4: 0.0087 and wild-type versus SNF7: 0.0116 (f); wild-type versus SDS: <0.0001, wild-type versus SDS+VPS4: 0.0085 and wild-type versus SDS+SNF7: 0.0394 (g). In e–g, the median lifespan is indicated as a gray horizontal line. WT, wild-type.
