Extended Data Fig. 6: Reduced LDLR hinders DHEA-S secretion in adrenocortical cells.
From: Aging induces region-specific dysregulation of hormone synthesis in the primate adrenal gland
(a) Western blot images (left) and quantification (right) showing LDLR protein level in young (n = 4), middle-aged (n = 3) and old (n = 4) cynomolgus monkey adrenal gland tissues. (b) GSEA enrichment curves show no significant change in the aging-related pathway after LDLR knockout in NCI-H295R cell. (c) Volcano plot showing the results of differential expression analysis by bulk RNA-seq in NCI-H295R cells transfected with CRISPRko sg-NTC and sg-LDLR lentivirus. Each dot represents a gene. For the western blot analysis, in a, GAPDH is used as the loading control. For bar graphs, data are presented as mean ± s.e.m. b, c, n = 4 biological replicates. Statistical significance is determined in a, P value by two-tailed unpaired Student’s t-test.
