Fig. 7: Apoptotic neurons upregulate lysosomal genes in microglia. | Nature Aging

Fig. 7: Apoptotic neurons upregulate lysosomal genes in microglia.

From: Single-nucleus sequencing reveals enriched expression of genetic risk factors in extratelencephalic neurons sensitive to degeneration in ALS

Fig. 7: Apoptotic neurons upregulate lysosomal genes in microglia.

a, Diagram of microglia and neuronal differentiation from PSCs and induction of apoptosis neurons and feeding to iMGLs (piNs, patterned induced neurons). b, Brightfield images of untreated day 40 iMGLs and day 40 iMGLs that were fed apoptotic neurons for 24 h. c. RT–qPCR quantification of homeostatic and DAM genes after feeding (AN, apoptotic neurons). d, RT–qPCR quantification of selected ALS–FTD-associated and lysosomal genes 24 h after feeding iMGLs with apoptotic neurons (AN, apoptotic neurons). (t-test, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3 biological replicates). piNS, induced neurons from hPS cells.

Back to article page