Extended Data Fig. 8: CD38 is upregulated in HSCs during aging.
From: An NAD+-dependent metabolic checkpoint regulates hematopoietic stem cell activation and aging
a. Schematic illustration of the NAD+ metabolic pathways. b-g. Comparison of HSCs isolated from young (3 months old) and old (24 months old) WT mice. B. Quantitative real-time PCR analyses of NAD+ metabolic enzymes (PARP1, p = 0.4431; NAMPT, p = 0.1517; NAPRT, p = 0.1546; NMNAT3, p = 0.3542; QPRT, p = 0.0419; CD38, p = 0.0010). n = 6 and 7 (CD38). n = 3 and 4 (other enzymes). C, D. Representative histograms (C) and quantification (D) for CD38 staining analyzed via flow cytometry (p = 0.0472). n = 4. E-G. Flow cytometry analyses of Fluo-4 (p = 0.0269) (E), TMRM (p = 0.0005) (F), and ROS (p = 0.0001) (G) staining. n = 5. Data are presented as mean values +/- SE. *: p < 0.05. **: p < 0.01. ***: p < 0.001. ns: p > 0.05. One-sided student’s t test.
