Extended Data Fig. 9: No observable mitophagy in the ND1 mutant oocytes.
(a) Heatmap presenting detailed gene expression profiles related to mitophagy pathway from Stero-seq analysis, log-normalized for clarity using Seurat. (b) Representative TEM image of oocytes in secondary follicles from ND1 and WT mice. Four distinct views from each mouse are presented. A zoomed-in view highlights a potential autophagic structure. (c) Quantitative analysis of autophagysome-like structures in (B). Data are mean ± SEM. n = 60 for WT; n = 71 for ND1; with p-value from two-tailed Mann Whitney test. (d) Representative images of LC3B and MTCO1 co-staining in oocytes in secondary follicle of WT and ND1 mutant mice. The experiment was repeated three times. (e) Representative images of LAMP1 and TOMM20 co-staining in oocytes in secondary follicle of WT and ND1 mutant mice. The experiment was repeated five times.
