Extended Data Fig. 6: Senescent features of tdTomato+ cells in PT mice skin after wound injury.
From: Clearance of p21 highly expressing senescent cells accelerates cutaneous wound healing
(a) Representative images of immunofluorescence staining of tdTomato of unwounded and wounded PT mice skins 2 days post injury. (b) The percentage of tdT+ cells per field (averaged from 3 random fields) in unwounded PT (n = 3) and in both the inside and outside wounded areas of wounded PT (n = 3) skins. (c) Representative images of immunofluorescence staining of tdTomato and γH2A.X of wounded PT skins 2 days post injury. (d) The number of γH2A.X foci in each tdT+ (n = 81) and tdT- cells (n = 267) from wounded PT skins 2 days post injury. (e) The proportion of tdT+ and tdT- cells with at least one γH2A.X foci in wounded PT mice skin samples (n = 3) 2 days post injury. (f-h) Representative images (f), cell size distribution (g) and average cell size of tdT+ and tdT- cells from five wounded PT skins 2 days post injury. The gating details can be found in the supplementary information file. Results were shown as means ± SEM. Two-tailed, unpaired Student’s t-test: b (PT-unwounded versus PT-wounded inside). Two-tailed, paired Student’s t-test: b (PT-wounded inside versus PT-wounded outside), e and h. Two-tailed, Mann-Whitney U test: d.
