Extended Data Fig. 7: Clearance of p21high cells accelerates wound healing in female mice.
From: Clearance of p21 highly expressing senescent cells accelerates cutaneous wound healing
(a) The wound closure curves of PT female mice treated with TAM (n = 8) or corn oil (n = 7) daily from day 1 to day 5 post-injury. Wound area was measured at the indicated days post injury as a percent of initial (Day 0) wound size. (b) The wound closure curve AUCs of corn oil treated PT (n = 7) and TAM treated PT (n = 8) female mice. (c) The wound closure curves of PT (n = 11) and PTD (n = 9) male mice treated with TAM daily from day 1 to day 5 post-injury. Wound area was measured at the indicated days post injury as a percent of initial (Day 0) wound size. (d) The wound closure curve AUCs of PT (n = 11) and PTD (n = 9) male mice. (e) The wound closure curve of PT (n = 16) and PTD (n = 15) female mice with TAM daily from day 1 to day 5 post-injury. Wound area was measured at the indicated days post injury as a percent of initial (Day 0) wound size. P value: Day 3 (0.0045), Day 4 (0.0043), Day 5 (0.0111), Day 7 (0.0269), Day 10 (0.0132). (f) The wound closure curve AUCs of PT (n = 16) and PTD (n = 15) female mice. (g) Bulk RNAseq was performed on PTD wound tissues (n = 5) and PT unwound tissues (n = 3) at the indicated time point. Enriched hallmark gene sets in wound PTD tissues versus unwound tissues with False Discovery Rate (FDR) < 0.05 are shown and considered as statistically significant. Results were shown as means ± SEM. * p < 0.05, ** p < 0.01. Two-tailed, unpaired Student’s t-test.
