Fig. 6: GD3 is a senescence-associated surface marker in bleomycin-induced fibrotic lungs. | Nature Aging

Fig. 6: GD3 is a senescence-associated surface marker in bleomycin-induced fibrotic lungs.

From: A ganglioside-based immune checkpoint enables senescent cells to evade immunosurveillance during aging

Fig. 6: GD3 is a senescence-associated surface marker in bleomycin-induced fibrotic lungs.

a, Quantification by flow cytometry of the frequency of SA-β-Gal+/− cells and GD3+/− cells in fibrosis-bearing lungs. b–d, Volcano plot (b), heatmap of DEGs (c) and KEGG pathway analysis (d) between sorted GD3+SA-β-Gal− and GD3+SA-β-Gal+ cells in bulk RNA-seq. Important senescence pathways or genes are highlighted or pinpointed in yellow. e,f, Senescence ssGSEA (e) and deconvolution analysis (f) of the GD3+/− and SA-β-Gal+/− sorted fractions. g, Characterization by ImageStreamX of senescent cells using SA-β-Gal assay, GD3, EPCAM and CD45 staining. Experiments were performed with n = 4 mice per group. *P < 0.05, two-tailed Mann–Whitney U-test.

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