Extended Data Fig. 2: Senescent cells but not their SASP affect immune infiltration and NK cell activation.
a, Gating strategy for the immune phenotyping of infiltrating cells from the Matrigel plug assay (Fig. 1a-b). b, Quantification of the immune cell infiltration induced by proliferative, oncogene or different stress-induced senescent MRC5 cells (left panel) or supernatant (right panel) in matrigel plugs. c-d, Quantification of the NK cell infiltration (c) and degranulation (d) induced by the supernatant from proliferative, replicative senescent, oncogene or stress induced MRC5 cells in matrigel plugs. e, Quantification of in vitro migration of primary human NK cells in presence of supernatant from pdl30 or bleomycin induced senescent MRC5 during transwell assays. f, Positive (YAC-1 cells, a strong NK cell target) and negative controls of degranulation (CD107a+ NK cells) or IFN-g production in in vitro co-culture experiments. g, Analysis of IFN- g production in in vitro co-culture experiments with senescent cells. All experiments are performed with n = 9 mice per group (a-d); *p < 0.05, **p < 0.01, and ***p < 0.001; Data are represented as mean ± s.e.m.; two-tailed Mann–Whitney U test or data represent the mean of n = 3 independent experiments; *p < 0.05, **p < 0.01, and ***p < 0.001; two-tailed Mann–Whitney U test (e, f).
