Extended Data Fig. 1: Age associated changes in feeding behavior and eye morphology.
From: Regeneration leads to global tissue rejuvenation in aging sexual planarians
(a) Average time spent on food in four independent feeding sessions for young (8MO) and old (18MO) planarians. Each dot represents the average time from 8 animals that were examined independently in one feeding session. Two-sided Welch Two Sample t-test. (b) The amount of liver ingested per feeding session, normalized by the weight of the animal (mg/mg). Each dot represents one animal. Young, 7MO: 7 animals. Old, 12MO: 6 animals. Red and cyan represent two feeding sessions of the same animals. (c) Development of EEP phenotypes over time in three animals from 4MO to 8MO. From top to bottom: ectopic eye, ectopic eye pigment, duplicated eyes. wks = weeks-old (d) Body size changes over time in 44 individually housed animals, from 4MO to 8MO. Red lines: animals developed early signs of EEP. Grey lines: animals with normal eyes. *: the first time point when EEP was visible. #: the first time point when EEP was visible, and the animal was at its largest size. w4, w5, w6…w42: worm IDs, corresponding to eye phenotypes in source data. (e-f) No significant differences in the timing of EEP appearance (e) or body sizes of EEP appearance (f) between ectopic eyes and ectopic pigment cells. Each data point represents an individual with EEP, n = 10 individuals were examined. Welch two sample t-test. (a-b, e-f) The boxplots show the median (center), IQR (box) and whiskers extending to 1.5 × IQR.
