Extended Data Fig. 3: Reanalysis of publicly available scRNAseq data identifies kTregs with a pro-inflammatory signature.
From: KLRG1 identifies regulatory T cells with mitochondrial alterations that accumulate with aging
(a) UMAP representation and clustering of a new analysis of publicly available scRNAseq data from CD4+ T cells from young and old mice. Clusters are colored as naïve (blue), rTregs (yellow), aTregs (orange), kTregs (red), TEM (light green), cytotoxic (brown) and exhausted (dark green). (b) Quantification of the percentage of kTreg cells in young and old mice (n = 4 mice per group). (c) Comparative expression of genes related to Treg function in aTreg and kTreg (n = 4 mice per group). (d) Violin plots showing the expression of several pro-inflammatory markers in each cluster of CD4+ T cells (n = 4 mice per group). Each dot represents an individual mouse. Box-and-whisker plots show the median, the maximum, the minimum and 25th and 75th percentiles. Statistical analysis was performed using two-tailed unpaired Welch’s t test (b). Exact P values and additional statistical parameters can be found in the source data.
