Extended Data Fig. 8: Mitochondrial H2O2 suppresses Sirt1 in ECs.
a, Schematic diagram of the experimental setting: PECs were transduced with lentiviral vector encoding DAAO-mito, followed by treatment with D-alanine (10 mM) for 72 h for generation of H2O2 in cell mitochondria. b, Immunoblots (Left) and quantitative plots (Right) demonstrate that mitochondrial H2O2 significantly reduces Sirt1 expression in PECs (n = 5–6). Error bars represent SD (b). Data represent triplicated biologically independent experiments. P values were calculated using a two-tailed unpaired Student’s t-test (b). Images created with BioRender.com (a). (*P < 0.05). Source data are provided as a Source Data file.
