Extended Data Fig. 9: Acetate regulates Nrf2 by Sirt1 modulation in ECs.
a, Schematic diagram of the experimental setting: PECs were transfected with siNeg or siSirt1 (for 24 h) in the presence of PAA plus sodium acetate (10 μM + 3 μM, for 72 h). b, Immunoblots (Left) and quantitative plots (Right) show that silencing of Sirt1 (by siSirt1) significantly reduces the expression of Sirt1 and Nrf2 (deacetylated form) in the nuclei of PECs treated with PAA plus sodium acetate compared to those transfected with siNeg (n = 6). Error bars represent SD (b). Data represent triplicated biologically independent experiments. P values were calculated using a two-tailed unpaired Student’s t-test (b). Images created with BioRender.com (a). (*P < 0.05, **P < 0.01). Source data are provided as a Source Data file.
