Extended Data Fig. 10: Acetate-mediated SASP downregulation is dependent on Sirt1 in ECs.
a, Schematic diagram of the experimental setting: PECs were transfected with siNeg, siSirt1, or siNFκB (for 24 h) in the presence of PAA plus sodium acetate (10 μM + 3 μM, for 72 h) or PAA alone (10 μM, for 72 h). b, Immunoblots (top) and quantitative plots (bottom) demonstrate that Sirt1 knockdown markedly increases NFκB acetylation at Lys310 in PECs incubated with PAA plus sodium acetate compared to siNeg-transfected counterparts (n = 6). c, Immunoblots show that the SASP component IL-6 is downregulated in siNFκB-transfected PECs in the presence of PAA (n = 6). Error bars represent SD (b,c). Data represent triplicated biologically independent experiments. P values were calculated using a two-tailed unpaired Student’s t-test (b,c). Images created with BioRender.com (a). (**P < 0.01, ***P < 0.001). Source data are provided as a Source Data file.
