Fig. 1: Progerin is recurrently expressed in up to 8.1% of the VSMCs in CKD arteries.
From: Recurrent somatic mutation and progerin expression in early vascular aging of chronic kidney disease
a, Colocalization staining of progerin (red), CD31 (white) and αSMA (green) in CKD arteries. White arrowheads indicate progerin-positive cells. This staining was performed on five independent CKD arteries. b, Immunostaining on CKD arteries showing both isolated (a) and clustered (b) progerin-positive cells (red). c, Quantification of progerin-expressing cells in controls (n = 23), CVD controls (n = 10) and patients with CKD (n = 50) (CKD versus Ctrls: P = 3 × 10−6; CKD versus CVD Ctrls: P = 0.0012). d, Graph showing the copy number of progerin transcripts normalized to GAPDH, in controls (n = 5) and CKD (n = 18) arteries (P = 0.0003). e, Graph showing that the frequency of progerin-positive cells did not correlate with age at sampling. Scale bars, 20 μm (a), 50 μm (b(i)), 10 μm (b(ii)). Statistics were Kruskal–Wallis test with Dunn’s correction for multiple comparisons (c), Mann–Whitney test with a two-tailed 95% confidence interval (d), Spearman correlation coefficients with a two-tailed 95% confidence interval for the Ctrls (n = 23) and CVD Ctrls (n = 10) groups, and Pearson correlation coefficients with a two-tailed 95% confidence interval for the CKD group (n = 50) (e). Data are presented as mean values ± s.e.m. (c,d). **P < 0.01; ***P < 0.001.
