Extended Data Fig. 6: Accumulation of progerin does not interfere with the clonal propagation capacity of progerin-expressing cells.
From: Recurrent somatic mutation and progerin expression in early vascular aging of chronic kidney disease
(A) Colocalization pictures of Ki67 (red) and αSMA (green) in aortas of wild-type mice at P3, P8, P14 and P21. (B) Colocalization pictures of Progerin (red) and αSMA (green) in aortas of a 5-week-old uninjected Myh11:Confetti:Lmna1827T mouse. (C) Graph comparing the frequency of Ki67-positive cells in the media of Myh11:Confetti:Lmna1827T/+ and Myh11:Confetti:Lmna1827T/1827T mice at P8 and P21. (D) Graph showing the frequency of confetti-positive cells in the media of Myh11:Confetti:Lmna1827T/+ and Myh11:Confetti:Lmna1827T/1827T mice at P8 and P21. (E) Graph showing the frequency of confetti cells forming clusters in the media of Myh11:Confetti:Lmna1827T/+ and Myh11:Confetti:Lmna1827T/1827T mice at P8 and P21. Scale bars: A, B = 25μm. Statistics: C-E: Number of mice: Myh11:Confetti:Lmna1827T/+ P8: n = 5, P21: n = 7; Myh11:Confetti:Lmna1827T/1827T P8: n = 9, P21: n = 10. Two-way ANOVA with Tukey’s correction for multiple comparisons. Data presented as mean values ± SEM.
