Fig. 3: Progerin-expressing cells form VSMC clusters in CKD arteries.
From: Recurrent somatic mutation and progerin expression in early vascular aging of chronic kidney disease
a, Immunofluorescent image showing the distribution of progerin-positive cells (green/highlighted in pink) in a CKD artery. Cell clusters are defined by yellow-/blue-dashed circles. Clusters were visualized in 21 of 32 independent CKD arteries. Scale bar, 100 μm. b, Spatial reconstruction based on serial artery sections showed progerin-positive cells (red) in the medial layer of a CKD artery. c, Graph showing the frequency of progerin-positive cells that form clusters for each CKD artery (n = 26). d, Graph showing the frequency of clusters that are formed by 2, 3, 4 or ≥5 progerin-positive cells per CKD artery (n = 21). e,f, Graphs showing the positive correlation between the frequency of progerin-expressing cells and the total number of clusters/sample (n = 26 CKD) (e) or the number of larger clusters/sample (n = 26 CKD) (f). g, Graph representing the positive correlation between the FA of the LMNA c.1824C>T mutation and the frequency of clusters containing ≥5 cells (n = 25 CKD). Statistics were Spearman correlation coefficients with a two-tailed 95% confidence interval (e,f). Data are presented as mean values ± s.e.m. (c,d).
