Fig. 4: Progerin-expressing cells are positive for markers of proliferation in CKD arteries.
From: Recurrent somatic mutation and progerin expression in early vascular aging of chronic kidney disease
a, Analysis of proliferating cells in CKD (n = 15) and control (n = 4) arteries, as detected by staining for Ki67 (red) (P = 0.0279). b, Immunostaining against progerin (red), PCNA (white) and αSMA (green) showing a cluster of proliferative progerin-positive VSMCs in a CKD artery. White arrows point to that cluster. This staining was performed on three independent CKD arteries. c, Immunostaining against progerin (red) and PCNA (white) showing a cluster of progerin-expressing cells in CKD and control arteries. d, Graph representing the fold change of PCNA-positive cells in control (n = 9) versus CKD (n = 20) arteries (P = 0.0006). e, Circle plot showing the distribution of progerin-positive cells among the PCNA+53BP1− cells in the media layer of CKD arteries (s.d. ±12). f, Graph showing the correlation between the frequency of PCNA-positive cells and the frequency of progerin-positive cells. g, Graph representing the frequency of PCNA-positive cells present in progerin-negative and progerin-positive cells in CKD arteries (n = 12). h, Graph showing the frequency of PCNA only positive cells in progerin clusters (n = 9 CKD). Scale bars, 10 μm (a), 25 μm (b) and 50 μm (c). Statistics used were a nonparametric Mann–Whitney U-test with a two-tailed 95% confidence interval (a,d,g) and Spearman correlation coefficients with a two-tailed 95% confidence interval (f). Data are presented as mean ± s.e.m. (a,d,g,h). *P < 0.05; ***P < 0.001.
