Fig. 7: Progerin-expressing cells show molecular changes indicative of senescence in CKD arteries.
From: Recurrent somatic mutation and progerin expression in early vascular aging of chronic kidney disease
a, Colocalization staining of P21 (green) and progerin (red) in arteries from patients with CKD. b, Graph displaying the frequency of P21-positive cells in the arteries of Ctrls (n = 9) versus patients with CKD (n = 13) (P = 4.8 × 10−5). c, Circle plot illustrating the distribution of progerin-expressing cells among the P21-positive cells in the media layer of CKD arteries (s.d. ±17). d, Graph showing the correlation between the frequency of P21-positive cells and the frequency of progerin-positive cells (n = 9 Ctrls and 13 CKD). e, Graph representing the frequency of P21-positive cells present in progerin-negative and progerin-positive cells of CKD arteries (n = 13) (P = 1.9 × 10−7). f, Colocalization staining of P16 (green) and progerin (red) in arteries from patients with CKD. g, Graph displaying the frequency of P16-positive cells in the arteries of Ctrls (n = 10) versus patients with CKD (n = 16) (P = 0.0035). h, Circle plot illustrating the distribution of progerin-expressing cells among the P16-positive cells in the media layer of CKD arteries (s.d. ±7.5). i, Graph showing the correlation between the frequency of P16-positive cells and the frequency of progerin-positive cells (n = 10 Ctrls and 16 CKD). j, Graph representing the frequency of P16-positive cells present in progerin-negative and progerin-positive cells of CKD arteries (n = 16) (P = 0.0194). Scale bars, 25 μm (a,f). Statistics were nonparametric Mann–Whitney U-test with a two-tailed 95% confidence interval (b,e,g,j) and Spearman correlation coefficients with a two-tailed 95% confidence interval (d,i). Data are presented as mean ± s.e.m. (b,e,g,j). *P < 0.05; **P < 0.01; ***P < 0.001.
