Extended Data Fig. 4: hUSI revealed potential senescence regulators.
a. A high similarity transcript (ENSG00000275464) hinders the design of siRNA for the knockdown of PWP2. b-e. Senescence validation of PRIM1 knockdown in ARPE19 cells (n = 3 biological replicates; two-tailed t-test, the exact p values were shown in Source Data Extended Data Fig. 4). PRIM1 was knocked down by two siRNAs (si-PRIM1-1 and si-PRIM1-2) (b). The senescence status was demonstrated by representative images of SA-β-Gal staining (c). The gene expression of CDKN1A, CDK1, LMNB1 and MKI67 evaluated by qRT-PCR (n = 3 biological replicates) (d) and the Western blot of p21 protein levels (e) before and after PRIM1 knockdown in ARPE19 cells, with GAPDH serving as the internal control. f-i, j-m, n-q and r-u present the senescence validation results for PSMA7, INCENP, PSMD2, and DDX49 (as those in b-e), respectively. Data are presented as mean ± SEM. *, **, and *** denote p < 0.05, p < 0.01 and p < 1e-3, respectively, two-tailed t-test. The exact p values of f-h, j-l, n-p, r-t were shown in Source Data for Extended Data Fig. 4.
