Extended Data Fig. 6: The effects of EVsABPCs mitigate organ senescence in aged mice.
a,b, Representative images of Masson’s trichrome staining for liver (a) and kidney (b). Scale bar,100 μm (top of a and b) or 350 μm (bottom of a and b). c, Representative images of immunofluorescence staining of TUNEL in the kidney. Scale bar, 200 μm. d,e, Representative images of H&E (d) and Masson’s trichrome (e) staining for skin. Scale bar, 50 μm (d) or 100 μm (e). f, Representative immunofluorescence images of γ-H2AX in skin. Scale bar, 500 μm. g, Representative immunofluorescence images of γ-H2AX staining in brain. Scale bar, 100 μm. h,i, The percentage of collagen fiber area in liver (h) and kidney (i) measured by the Masson’s trichrome staining (n = 8). j, Quantitative analysis of expression level of TUNEL in kidney (n = 8). k–n, Quantitative analysis of epidermal thickness (k) and number of epidermal cells per millimeter thickness (l) measured by H&E staining, as well as dermal thickness (m) and collagen content (n) measured by the Masson’s trichrome staining (n = 8). o, Quantitative analysis of expression level of γ-H2AX in skin (n = 8). p, Quantitative analysis of expression level of γ-H2AX in brain (n = 8). q, Clustering trend plots showed the numbers of DEGs across different organs. r, Cluster analysis showed the main upregulated DEGs in liver, kidney, skin, intestine, brain and pan-tissues treated with EVsABPCs. s–u, Representative tracking images of Y maze (s), NOR (t), and EPM (u) in EVsA-BMSCs and EVsF-BMSCs groups. v, Ex vivo fluorescence images of brains at 48 h after injection of DiR-labeled EVs by tail vein and their quantitative analysis (n = 8). Scale bar, 500 μm. Statistical significance was calculated by one-way ANOVA with Bonferroni’s multiple comparisons test (h–p and v). Data were presented as mean ± s.d. ***P < 0.001, and ****P < 0.0001.
