Fig. 5: NCOA7 promotes SG clearance via autophagy after stress removal.
From: Stress granule clearance mediated by V-ATPase-interacting protein NCOA7 mitigates ovarian aging
a, Immunofluorescence and quantification of SGs in GCs from control, POI and middle-aged participants (n = 15 views per group). Scale bar, 5 μm. P values (from left to right): ****P < 0.0001, ****P < 0.0001, ****P < 0.0001, ****P < 0.0001, ****P < 0.0001, ****P < 0.0001. b, Immunofluorescence of SGs (marked by G3BP1 and CAPRIN1) in WT, NCOA7-KO, NCOA7-rescued and NCOA7 domain-truncated KGN cells after treatment with arsenate and subsequent recovery for the indicated time periods. Scale bar, 5 μm. c, A His pulldown assay of NCOA7–Flag from arsenate-treated cells using His–LC3 proteins. d, Immunofluorescence and quantification of SGs in KGN cells treated with DMSO or chloroquine (CQ) (n = 16 views in quantification of stressed cells; n = 50 views per group in quantification of SGs per cell). Scale bar, 10 μm. ****P < 0.0001, **P = 0.0094. e, Immunofluorescence and quantification of SGs in ATG7-, ATG9A- and ATG13-knockdown KGN cells (n = 16, 16, 9 and 15 views, respectively in quantification of stressed cells; n = 33 views per group in quantification of SGs per cell). Scale bar, 10 μm. **P = 0.0065, **P = 0.0043, **P = 0.0045, ****P < 0.0001; NS, P = 0.1502; ****P < 0.0001. Data in a,d,e are expressed as mean ± s.e.m. and a one-way ANOVA with Dunnett’s post hoc test (a,e) and a two-tailed Student’s t-test (d) were used for group comparisons.
