Fig. 7: Clinical alleviation of ovarian aging by promoting autophagy or targeting NCOA7 to attenuate GC senescence.
From: Stress granule clearance mediated by V-ATPase-interacting protein NCOA7 mitigates ovarian aging
a,b, Immunofluorescence (a) and quantification (b) of SGs (n = 5 biological replicates per group) in GCs from control, POI and middle-aged participants treated with DMSO or rapamycin. Scale bars, 20 μm or 5 μm. P values (from left to right): ***P = 0.0001, ***P = 0.0005, ***P = 0.0007, ***P = 0.0006. c, Quantification of SA-β-gal-positive cells in GCs from POI (n = 5 biological replicates) and middle-aged (n = 4 biological replicates) participants treated with DMSO or rapamycin. ****P < 0.0001, ****P < 0.0001, **P = 0.0061, ****P < 0.0001. d, Expression levels of senescence markers (p16INK4a and p21Cip1) in GCs from control, POI and middle-aged participants treated with DMSO or rapamycin (n = 4 biological replicates per group). NS, P = 0.8945; **P = 0.0059; *P = 0.0201; NS, P = 0.1368; **P = 0.0024; ****P < 0.0001. e, Analysis of E2 levels in the supernatant of GCs from control, POI and middle-aged participants treated with DMSO or rapamycin (n = 4 biological replicates per group). *P = 0.0494, ****P < 0.0001, **P = 0.0013. f, Quantification of SGs in GCs from control, POI and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs (n = 5 biological replicates per group). NS, P = 0.6915; **P = 0.0004; *P = 0.0132; NS, P = 0.3466; *P = 0.0104; *P = 0.0143. g, Quantification of expression levels of senescence markers (p16INK4a and p21Cip1) in GCs from control, POI and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs (n = 4 biological replicates per group). NS, P = 0.7277; **P = 0.0047; ***P = 0.0006; NS, P = 0.9957; ***P = 0.0005; ***P = 0.0003. h, SA-β-gal staining and the percentage of SA-β-gal-positive cells in GCs from POI and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs (n = 5 biological replicates per group). Scale bar, 20 μm. ****P < 0.0001, ****P < 0.0001, *P = 0.0150, ***P = 0.0002. Data in b–h are expressed as mean ± s.e.m. and compared by two-tailed Student’s t-test.
