Extended Data Fig. 4: Proximity labeling-based identification of mRNAs and proteins associated with LARP-1.
From: Mitochondria-associated condensates maintain mitochondrial homeostasis and promote lifespan
a, Schematic depiction of proximity labeling and identification of mRNAs and proteins associated with LARP-1. Proximity labeling was conducted with LARP-1::APEX2 animals, and LARP-1::GFP-expressing animals were used as control. b, Graphic summary of data reproducibility (mRNAs) derived from the control (LARP-1::GFP) and LARP-1::APEX2 samples. 3 repeats were performed for each group. c, Graphic summary of LARP-1::APEX2-enriched mRNAs encoding proteins of different intracellular organelles. d. Comparison of LARP-1::APEX2-enriched mRNAs encoding mitochondrial proteins with down-regulated mitochondrial protein identified by LC-MS in larp-1 mutants. e-g, qPCR analysis of the levels of mRNAs encoding the indicated protein subunits of MICOS complex (e), respiratory complex I (f), and ATP synthase complex (g)in N2, larp-1(yq421) and larp-1(yq565) animals. n = 3 independent experiments. h, Western-blotting analysis of LARP-1::APEX2-enriched proteins with Streptavidin-HRP antibody. i, Representative heatmaps of RNA-binding proteins enriched by LARP-1::APEX2. Triplicate samples were analyzed for each group. Data are graphed as mean ± s.e.m. P values were determined using one-way ANOVA (e-g). NS, not significant (P > 0.05). Exact P values are shown in SourceData ED Fig 4 (e-g).
