Extended Data Fig. 6: Whole mount immunofluorescence confocal microscopy of CD169⁺ NAMs in VAT.

(a) Whole mount immunofluorescence confocal microscopy of visceral adipose tissue (VAT) from adult female CD169:mTmG dual reporter mice labeled with the pan-neuronal marker TUBB3 (merged image, top left). CD169 is labeled with membrane eGFP (mGFP⁺) (green, top right), non-recombined structures such as adipocytes, nerves, and blood vessels are labeled with membrane Tomato (mTomato⁺) (red, bottom left), and nerves are labeled with TUBB3 (blue, bottom right). (b) Whole mount immunofluorescence confocal microscopy of visceral (VAT, left), subcutaneous (SAT, middle), and brown adipose tissue (BAT, right) from adult female CD169:mTmG dual reporter mice labeled with the pan-neuronal marker TUBB3 (merged image, top left). CD169 is labeled with membrane eGFP (mGFP⁺) (green, top right), non-recombined structures such as adipocytes, nerves, and blood vessels are labeled with membrane Tomato (mTomato⁺) (red, bottom left), and nerves are labeled with TUBB3 (blue, bottom right).(c) Whole mount immunofluorescence confocal microscopy of visceral adipose tissue (VAT) from adult female CD169:mTmG dual reporter mice labeled with the pan-neuronal marker TUBB3 (merged image, top left). CD169 is labeled with membrane eGFP (mGFP⁺) (green, top right), non-recombined structures such as adipocytes, nerves, and blood vessels are labeled with membrane Tomato (mTomato⁺) (red, bottom left), and nerves are labeled with TUBB3 (blue, bottom right).