Extended Data Fig. 7: Gating strategy to quantify the depletion efficiency of NAMs in VAT. | Nature Aging

Extended Data Fig. 7: Gating strategy to quantify the depletion efficiency of NAMs in VAT.

From: Nerve-associated macrophages control adipose homeostasis across lifespan and restrain age-related inflammation

Extended Data Fig. 7: Gating strategy to quantify the depletion efficiency of NAMs in VAT.

Schematic depicting the flow cytometry gating strategy used to quantify the depletion efficiency of nerve-associated macrophages (NAMs) and other immune cell subsets in the visceral white adipose tissue (VAT) and spleen from wild-type (WT) and CD169-DTR mice following intraperitoneal diphtheria toxin administration in 3-, 6-, 9-, and 24-month-old female mice and at the following endpoints: day 8, day 13, day 25, and day 34.

Back to article page