Fig. 2: Aging remodels resident adipose tissue macrophages.

a, UMAP representation of unsupervised clustering of resident F4/80⁺CD11b⁺ (CD45⁺CD45.2iv⁻CD3⁻CD19⁻SiglecF⁻) cells sorted from the VAT of young (2-month-old; 7,558 cells) and aged (21-month-old; 7,139 cells) male and female mice. b, Repartition of cells in each cluster as a fraction of total cells (male mice and female mice) from young and aged VAT. c, Violin plots depicting the expression of Siglec1, Itgax, Cd226, Cd9, Cd163, Folr2 and Cd38 genes by cluster. d, Schematic summarizing the cell surface markers used to define seven clusters identified via scRNA-seq of resident F4/80⁺CD11b⁺ cells (CD45⁺CD45.2iv⁻CD3⁻CD19⁻SiglecF⁻) from the VAT. Abbreviations for each subset and defining cell surface markers are as follows: NAM (C5): CD169⁺CD11c⁻; MP-1 (C13): CD169⁺CD11c⁺; VAMs (C0): CD38⁺FOLR2⁺CD169⁻CD11c⁻; AAMs (C4): CD38⁺FOLR2⁻CD169⁻CD11c⁻; LAMs (C10): CD9⁺CD11c⁺CD226⁻CD163⁻CD169⁻; adipose tissue macrophages-2 (ATM-2, C3): CD226⁺CD11c⁺CD163⁻CD169⁻; and DC-1 (C6): CD163⁺CD226⁺CD11c⁺CD163⁺CD169. e, Representative flow cytometry plots depicting the proportion of NAM, MP-1, VAM, AAM, LAM, ATM-2 and DC-1 populations in the VAT SVF isolated from 3-month and 22-month-old male and female mice. f, Quantification of the proportion of NAM, MP-1, VAM, AAM, LAM, ATM-2 and DC-1 populations in the VAT SVF from 3- and 22-month-old male and female (n = 5 per sex and group) mice. Data are displayed as mean ± s.e.m. and are representative of one independent experiment. Statistical significance was determined via a Student’s two-tailed t-test with α = 0.05. Results were considered statistically significant as *P < 0.05; **P < 0.01; ****P < 0.0001; NS, not significant.