Extended Data Fig. 2: Single cell RNA-sequencing of resident F4/80+CD11b+ cells from the VAT of young and aged males and females identified 16 distinct clusters. | Nature Aging

Extended Data Fig. 2: Single cell RNA-sequencing of resident F4/80+CD11b+ cells from the VAT of young and aged males and females identified 16 distinct clusters.

From: Nerve-associated macrophages control adipose homeostasis across lifespan and restrain age-related inflammation

Extended Data Fig. 2: Single cell RNA-sequencing of resident F4/80+CD11b+ cells from the VAT of young and aged males and females identified 16 distinct clusters.

(a) Uniform Manifold Approximation and Projection (UMAP) representation of unsupervised clustering of 15,709 resident F4/80+CD11b+ (CD45+CD45.2iv−CD3−CD19−SiglecF−) cells sorted from VAT of young (2-month-old) and aged (21-month-old) male (13,134 cells) and female mice (2,575 cells) (top) and repartition of cells in each cluster as a fraction of total cells by cluster (bottom). (b) Heatmap depicting the top 25 differentially expressed genes (DEGs) per cluster. (c) Uniform Manifold Approximation and Projection (UMAP) (left) and violin plots (right) showing the expression of Ptprc (CD45), Itgam (CD11b), and Adgre1 (F4/80) genes by cluster.

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