Extended Data Fig. 8: DANA counteract E-motif-induced systemic aging.
From: Elastin-derived extracellular matrix fragments drive aging through innate immune activation
a, Diagram illustrating cell transplantation using mice injected with E-motif or E-motif+DANA as donors. b, Survival curves for Rag mice after adoptively transferred with T/B cells from Neu1−/− mice treated with or without E-motif (Rag+Neu1−/−vehicle-T/B: n = 9, Rag+Neu1−/−E-motif-T/B: n = 10, no significant difference between Rag+Neu1−/−E-motif-T/B and Rag+Neu1−/−vehicle-T/B). c, Illustrative representation of three groups of different treatment (vehicle, E-motif, and E-motif combined with DANA) over a 16-week period to verify the roles of DANA. Illustration was created using FigDraw. d, Body weight changes of mice treatment with E-motif, E-motif+DANA or vehicle over time (n = 6 per group; E-motif versus vehicle P = 0.0079, E-motif+DANA versus E-motif P = 0.0297). e, The ratio of T/B cells in peripheral blood (n = 6 per group, B cells: E-motif versus vehicle P = 0.0218, E-motif+DANA versus E-motif P = 0.0097; T cells: E-motif versus vehicle P = 0.0069, E-motif+DANA versus E-motif P = 0.0173). f,g, Serum levels of ALT, AST, IL-1 and MCP-1 (n = 6 per group, ALT: E-motif versus vehicle P = 0.0028, E-motif+DANA versus E-motif P = 0.0092; AST: E-motif versus vehicle P = 0.0040, E-motif+DANA versus E-motif P = 0.0170; IL-1: E-motif versus vehicle P = 0.0005, E-motif+DANA versus E-motif P = 0.0013; MCP-1: E-motif versus vehicle P = 0.0022, E-motif+DANA versus E-motif P = 0.0072). h, Survival curves for mice after cell transplantation using mice injected with E-motif or E-motif+DANA as donors (Rag-T/B and Rag+E-motif-DANA-T/B: n = 9 per group, Rag+E-motif-T/B: n = 8; Rag+E-motif-T/B versus Rag-T/B P = 0.0061 by log-rank test and P = 0.0025 by Gehan–Breslow–Wilcoxon test, Rag+E-motif-T/B versus Rag+E-motif-DANA-T/B P = 0.0279 by log-rank test and P = 0.0172 by Gehan–Breslow–Wilcoxon test). i, Serum levels of ELN fragments (n = 6, E-motif+DANA versus E-motif P = 0.0364). * P < 0.05, ** P < 0.01, *** P < 0.001. Unpaired two-tailed Student’s t-test with a two-tailed 95% confidence interval (e-g). Log-rank test and Gehan–Breslow–Wilcoxon test (b,h). Two-way ANOVA (d,i).
