Extended Data Fig. 2: CtBP2 interacts with HECT ligases to be monoubiquitinated, a sorting signal for the exosome secretion pathway.
From: The secreted metabolite sensor CtBP2 links metabolism to healthy lifespan
a. A Venn diagram showing the screening process to identify proteins with the dual characteristics of CtBP2 binding capability and residence in exosomes. b. CtBP2 and HECT ligases were exogenously expressed in HEK293 cells, and their interactions were determined. Domain structures of HECT ligases are depicted as rectangles in different colors above the blots (orange: C2 domain, black: WW domain, blue: HECT domain, X in red: PXDL motifs mutated in the mutants of HECT ligases (Mut)). c. Interactions between CtBP2 and SMURF1 or WWP2 at the protein level using purified recombinant proteins. d. Knockdown efficiencies of our siRNAs (n = 4, technical replicates, p < 0.0001). Data are expressed as mean ± SEM. ** denotes p < 0.01 analyzed by two-sided Student’s t-test (d).
