Fig. 1: REC8−FKBP12F36V−mClover3 knockin mice enable high-resolution live imaging of endogenous REC8 dynamics during oocyte meiosis.
From: A versatile cohesion manipulation system probes female reproductive age-related egg aneuploidy
a, Representative confocal immunofluorescence microscopy images of REC8 (marked with GFP antibodies) and chromosomes (Hoechst) in metaphase I-stage oocytes of a REC8−FKBP12F36V−mClover3 mouse. Single confocal sections spaced 1.5 µm apart are shown. b, Images from a representative high-resolution time-lapse movie of REC8−FKBP12F36V−mClover3 (endogenous REC8) and chromosomes (SiR-5-Hoechst) showing chromosome arm-specific removal of REC8 cohesin (and its retention at centromeric regions) during anaphase of meiosis I. c, Quantification of the number of anaphase I lagging chromosomes per cell in wild-type and REC8−FKBP12F36V−mClover3 oocytes. Data points represent individual oocytes. d, Quantification of the proportion of oocytes with zero or more anaphase I lagging chromosomes in wild-type and REC8−FKBP12F36V−mClover3 oocytes. e, Quantification of the proportion of oocytes that completed meiosis within 16 hours of GVBD in wild-type and REC8−FKBP12F36V−mClover3. Data are from three independent experiments. Statistical significance was evaluated using Student’s t-test (c–e). The number of analyzed oocytes is indicated in brackets and italics. GVBD, germinal vesicle breakdown; N.S., non-significant values.
