Fig. 4: Premature sister chromatid separation arising from PROTAC-mediated REC8 cohesin degradation is accelerated by actin cytoskeleton disruption.
From: A versatile cohesion manipulation system probes female reproductive age-related egg aneuploidy
a, Images from representative time-lapse movies of sister chromatids (H2B−mScarlet) in DMSO-treated, dTAG-13-treated, Cytochalasin D (CytoD)-treated or dTAG-13-treated and CytoD-treated eggs matured in vitro from REC8−FKBP12F36V−mClover3 oocytes. t = 0 minutes denotes the start of live imaging experiment. b, Quantification of the number of single chromatids per egg at 90 minutes of live imaging in DMSO-treated, dTAG-13-treated, CytoD-treated or dTAG-13-treated and CytoD-treated eggs matured in vitro from REC8−FKBP12F36V−mClover3 oocytes. c, Quantification of the proportion of cells containing single chromatids in DMSO-treated, dTAG13-treated, CytoD-treated or dTAG-13-treated and CytoD-treated eggs matured in vitro from REC8−FKBP12F36V−mClover3 oocytes. d, Measurements of chromatid scattering volumes from time-lapse movies of DMSO-treated, dTAG-13-treated, CytoD-treated or dTAG-13-treated and CytoD-treated eggs matured in vitro from REC8−FKBP12F36V−mClover3 oocytes. Lines represent mean values, and error bars indicate s.e.m. Data are from three independent experiments. Statistical significance was assessed using Student’s t-test (b,c) or two-way ANOVA (d). The number of analyzed oocytes is provided in brackets and italics. N.S., non-significant values.
