Extended Data Fig. 6: Quantifying human aging via S-FLIM of living blood cells.
From: Fluorescence lifetime clocks quantify senescence and aging
a,b, FLIM images (a) and fluorescence lifetime statistics (b) of neutrophils (incubated with FLP1 10 μM for 1 h) of 29 volunteers with different ages. Data are presented as mean values ± s.d. from biologically independent human for each group (n = 11 for young group, n = 10 for middle aged group, n = 8 for aged group). The fluorescence lifetime of neutrophils gradually decreases with the age of volunteers. p = 2.97 × 10−7 for young-middle aged, p = 5.29 × 10−6 for middle aged-aged, p = 1.40 × 10−8 for young-aged. Statistical significance was calculated using two tailed t-test. ****P<0.0001.
