Fig. 2: Impaired muscle regeneration results in FAP expansion in iEzh2^−/− mice.

a, Pdgfrα and Laminin immunostaining of TA muscle sections from control and iEzh2^−/− mice at 28 dpi. DAPI marks nuclei. Quantification of Pdgfrα⁺ cells is shown on the right. Two muscle sections per mouse were analyzed. Data are presented as mean ± s.d., n = 4 mice, ^*P = 0.0129. b, Breeding schematic for Pax7^creER and iEzh2^fl/fl mice crossed with PDGFRα^EGFP mice. c,d, Laminin immunostaining of TA muscle sections from control PDGFRα^EGFP or iEzh2^−/−;PDGFRα^EGFP mice at 7 dpi (c) and 28 dpi (d). GFP⁺ nuclei identify FAPs; DAPI marks nuclei. Quantification of GFP⁺ cells is shown on the right for each timepoint (c,d). Two muscle sections per mouse were analyzed. Data are presented as mean ± s.d. c, n = 9 mice, ^****P < 0.0001; d, n = 5 mice, ^**P = 0.004. e, EdU immunostaining of TA muscle sections from control PDGFRα^EGFP or iEzh2^−/−;PDGFRα^EGFP mice at 4 dpi. GFP⁺ nuclei identify FAPs. Quantification of GFP⁺EdU⁺ cells is shown on the right. Two muscle sections per mouse were analyzed. Data are presented as mean ± s.d., n = 4 mice,^***P = 0.0002. Two-tailed unpaired t-test was used for statistical comparisons in a and c−e. Lam, Laminin.

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