Fig. 3: FAPs isolated from iEzh2−/−;PDGFRαEGFP mice express pro-fibrotic transcripts.
a, Schematic of the scRNA-seq protocol. FAPs were FACS-isolated based on GFP expression. b, scRNA-seq UMAP plots of control PDGFRαEGFP and iEzh2−/−;PDGFRαEGFP mice showing FAP clusters. Clusters significantly modulated (>1.5 fold change) in iEzh2−/−;PDGFRαEGFP compared to controls are circled. Right panel shows a table with the relative cell percentages for each cluster. Two mice were employed per single scRNA-seq experiment, and the experiment was performed twice, resulting in a total sample size of n = 4 mice. A total of 10,857 cells were analyzed. c, Violin plots of fibrotic (left) and inflammatory (right) gene expression in the indicated clusters. d, Tenascin immunostaining of TA muscle sections from control PDGFRαEGFP or iEzh2−/−;PDGFRαEGFP mice at 7 dpi. Nuclear GFP reporter identifies FAPs; DAPI marks nuclei. Bottom panel shows quantification of Tnc+ cells in control PDGFRαEGFP or iEzh2−/−;PDGFRαEGFP mice. Two muscle sections per mouse were analyzed. Data are presented as mean ± s.d., two-tailed unpaired t-test, n = 3 mice,*P = 0.0353.
