Fig. 4: iEzh2−/− MuSCs promote FAP proliferation.
a, Schematic of FACS isolation and plating of MuSCs and FAPs. After 72 hours in culture, EdU assay and scRNA-seq were performed. b, EdU immunostaining on co-cultured MuSCs and GFP+ FAPs. MuSCs were isolated from either control PDGFRαEGFP (Ctrl MuSCs) or iEzh2−/−;PDGFRαEGFP (iEzh2−/− MuSCs) mice, whereas FAPs were isolated from control PDGFRαEGFP mice. Arrows indicate GFP+EdU+ cells. Right panel shows quantification of PDGFRαEGFP+EdU+ cells. Data are presented as mean ± s.d., two-tailed unpaired t-test, n = number of regions counted (n = 23), with cells pooled from three independent experiments **P = 0.0015. c, scRNA-seq UMAP plots of co-cultured MuSCs and FAPs isolated from control PDGFRαEGFP or iEzh2−/−;PDGFRαEGFP mice. Bar plots on the right show the relative percentage of cell clusters. d, Information flow showing context-specific signaling pathways enriched in co-culture conditions, with signaling directed from MuSCs to FAPs. e, RNA-seq tracks of IL-6 and Spp1 transcripts in control or iEzh2−/− MuSCs at 7 dpi and 3 dpi, respectively. f, scRNA-seq UMAP (from Fig. 3b) and violin plots showing transcripts expressed in the indicated clusters in control PDGFRαEGFP or iEzh2−/−;PDGFRαEGFP mice.
