Extended Data Fig. 9: Raptor deletion blocks the therapeutic effects of ALA and EDA in AD mice.
a, RaptorLoxP mice were crossed with hCD11b-CreERT2 knock-in and APP/PS1 mice, resulting in the APP/PS1/PAMARaptorERT2 mice. The mice were then administered with saline (lane C) or tamoxifen (lane T). Representative blots and a plot showing a selective deletion of Raptor protein in PAMAs. Data are presented as mean ± s.e.m., n = 3 mice per group. b, Representative blots and a plot showing that deletion of Raptor inactivates mTORC1 in PAMAs of AD mice. Data are presented as mean ± s.e.m., n = 3 mice per group. c, d, Representative images (c) and a plot (d) showing the areas of thioflavin-S-labeled amyloid plaques throughout the cortex and the hippocampus of PS1/PAMAsRaptor+/+ and PS1/PAMAsRaptor−/− mice at 8 months old of age. Data are presented as mean ± s.e.m., n = 12 slides from 4 mice per group. e, f, The latency (e, left) and the length (e, right) of swim path to reach a hidden platform during the training sessions and the representative heat maps (f, left) and the percentage (f, right) of time spent in searching of a hidden platform in targeting quadrant (quadrant 2) during the probe trial of the individual mice at 11 months old of age. In this study, the mice were treated with vehicle or A-E for 45 consecutive days. The behavioral tests were performed when the mice were at 11 months old of age. Data are presented as mean ± s.e.m., n = 10 mice per group. All experiments were repeated at least three times independently with similar results. One -way ANOVA with Bonferroni’s multiple comparisons test was used for a, b, d and f, and two-way ANOVA with Bonferroni’s multiple comparisons test was used in e. *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significant difference. The exact P values are reported as source data for Extended Data Fig. 9.
