Extended Data Fig. 3: Correlations between CENGEN and Calico datasets.
a) Amphid neurons are predicted to be significantly older than non-amphid neurons in the Calico day 1 dataset. Two-sided t-test p-value: 5.6e-08. b) Amphid neurons are predicted to be significantly older than non-amphid neurons in the CeNGEN dataset with a stochastic data-based clock. Two-sided t-test p-value: 7.2e-22. c) Amphid neurons express significantly more neuropeptides than non-amphid neurons. Two-sided t-test p-value: 5.95e-12. d) Amphid neurons express significantly more receptor genes than non-amphid neurons. Two-sided t-test p-value:8.45e-09. e) The number of neurotransmitters is not significantly different in amphid and non-amphid neurons. Two-sided t-test p-value: 0.94. f) The number of innexins is not significantly different in amphid and non-amphid neurons. Two-sided t-test p-value: 0.05. g) The number of expressed neuropeptides (y-axis) is significantly correlated (Pearson correlation 0.26, p-value 3e-03) with the predicted age by BitAge in the CeNGEN dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. h) The number of expressed receptor genes (y-axis) is significantly correlated (Pearson correlation 0.22, p-value 1.2e-02) with the predicted age by BitAge in the CeNGEN dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. i) The number of expressed neuropeptides (y-axis) is significantly correlated (Pearson correlation 0.36, p-value 2.9e-03) with the predicted age by BitAge in the Calico day 1 dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. j) The number of expressed receptor genes (y-axis) is significantly correlated (Pearson correlation 0.44, p-value 2.4e-04) with the predicted age by BitAge in the Calico day 1 dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. k) The number of expressed neuropeptides (y-axis) is significantly correlated (Pearson correlation 0.43, p-value 4.8e-07) with the predicted age by a stochastic data-based clock in the CeNGEN dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. l) The number of expressed receptor genes (y-axis) is significantly correlated (Pearson correlation 0.39, p-value 6.9e-06) with the predicted age by a stochastic data-based clock in the CeNGEN dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. m) Fraction-plot displaying the fraction of nematodes expressing neuronal volume markers in the ASI neuron categorized as ‘healthy’, ‘mildly damaged’, and ‘severely damaged’. Three to four cohorts were analysed, comprised of 10 – 30 individual nematodes, for every timepoint indicated. Kruskal-Wallis-test was employed to test for significant differences. n) The number of expressed innexin genes (y-axis) is significantly anti-correlated (Pearson correlation -0.19, p-value 3.7e-02) with the predicted age by BitAge in the CeNGEN dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. o) The number of expressed innexin genes (y-axis) is not-significantly anti-correlated (Pearson correlation -0.2, p-value 0.9e-01) with the predicted age by BitAge in the Calico day 1 dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. p) The number of expressed innexin genes (y-axis) is not-significantly anti-correlated (Pearson correlation -0.12, p-value 1.9e-01) with the predicted age by a stochastic data-based clock in the CeNGEN dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. q) The number of total synapses (y-axis) is not-significantly anti-correlated (Pearson correlation -0.04, p-value 6.6e-01) with the predicted age by BitAge in the CeNGEN dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. r) The number of total synapses (y-axis) is not-significantly anti-correlated (Pearson correlation -0.21, p-value 1.0e-01) with the predicted age by BitAge in the Calico day 1 dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. s) The number of total synapses (y-axis) is not-significantly anti-correlated (Pearson correlation -0.07, p-value 4.6e-01) with the predicted age by a stochastic data-based clock in the CeNGEN dataset. The regression model fit with a 95% confidence interval (shadowed area) is shown. t) Ciliated neurons are predicted to be significantly older than non-ciliated neurons in the Calico day 1 dataset. Two-sided t-test p-value: 4.4e-05. u) Ciliated neurons are predicted to be significantly older than non-ciliated neurons in the CeNGEN dataset with a stochastic data-based clock. Two-sided t-test p-value: 2.29e-14. v) Ciliated neurons are divided into 5 classes depending on where its cilia terminate. Neurons with exposed cilia are significantly older than non-ciliated neurons or neurons which cilia terminate in the cuticle or behind the cuticle in the Calico day 1 dataset (one-way ANOVA p-value: 2.68e-08, with a post-hoc Tukey test). w) Ciliated neurons are divided into 5 classes depending on where its cilia terminate. Neurons with exposed cilia are significantly older than non-ciliated neurons or neurons which cilia terminate in the cuticle or behind the cuticle in the CeNGEN dataset with a stochastic data-based clock (one-way ANOVA p-value: 6.6e-18, with a post-hoc Tukey test).
