Fig. 1: MERFISH paired with fluorescence immunohistchemistry allows for single microglia transcriptomics and imaging. | Nature Aging

Fig. 1: MERFISH paired with fluorescence immunohistchemistry allows for single microglia transcriptomics and imaging.

From: Simultaneous spatial transcriptomics and morphology profiling as tools to explore how microglia change with age

Fig. 1: MERFISH paired with fluorescence immunohistchemistry allows for single microglia transcriptomics and imaging.The alternative text for this image may have been generated using AI.

a, Schematic overview of the image processing pipeline to generate maximum-projected transcriptome decodings and images of microglia across the whole mouse brain. b, Neuronal and glial markers resolved in space. c, UMAP plots of microglia colored by identified clusters. d, UMAP plots of microglia (n = 3,970 cells) colored by marker gene expression. e, Representative images of IBA1-stained microglia from each of the identified clusters. Scale bars, 25 µm. f, Heatmap of average expression for each microglia cluster.

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