Fig. 3: Morphology−transcriptome correlation identifies compartment-enriched genes.

a, Schematic image of microglia among all other cells in the brain (top) and image of the same brain separated into the brain regions (bottom). b, Schematic representation of workflow for establishing correlation results. For every transcriptomic feature, we use a morphological feature on the same microglia to calculate the Spearman correlation (rs). c, Clustered heatmap showing the Spearman correlation between different microglial morphological features and select genes. d, Heatmap showing the ability of select genes to be able to cluster the cellsʼ morphological classifications. e, Genes among the top correlating genes for morphology characteristics in each region of the brain, sorted by number of regions in which they occur. f, Gene Ontology cell compartment terms for the genes that are among the top correlating genes in any segmented region of the brain. CC, Cellular Component; GO, Gene Ontology.