Extended Data Fig. 2: Secondary intrinsic excitability (IE) parameters in hippocampal excitatory neurons from E3-KI and E4-KI mice.
a-c, CA3 PC IE parameter values: resting membrane potential (RMP) (a), maximal firing rate (b), and firing accommodation (c). d-f, Type II DGC IE parameter values: RMP (d), maximal firing rate (e), and firing accommodation (f). g-i, CA1 PC IE parameter values: RMP (g), maximal firing rate (h), and firing accommodation (i). All data are represented as mean ± s.e.m. CA3 pyramidal cells (a-c): Young E3-KI: eight mice, n = 36 cells (35 in (c)). Young E4-KI: seven mice, n = 25 cells. Aged E3-KI: six mice, n = 24 cells (23 in (c)). Aged E4-KI: four mice, n = 21 cells. Dentate granule cells (d-f): Young E3-KI: four mice, n = 22 cells. Young E4-KI: three mice, n = 22 cells (21 in (d)). Aged E3-KI: five mice, n = 32 cells (31 in (d)). Aged E4-KI: five mice, n = 21 cells. CA1 pyramidal cells (g-i): Young E3-KI: four mice, n = 27 cells. Young E4-KI: four mice, n = 30 cells. Aged E3-KI: four mice, n = 30 cells (28 in (g)). Aged E4-KI: six mice, n = 21 cells. Significance was assessed using a maximum likelihood ratio test that included age and APOE genotype, with p-values corrected for multiple comparisons using the false discovery rate (5%). Post hoc two-tailed Student’s t-tests or Mann-Whitney U tests were performed between groups if significant genotype or age effects were observed.
