Extended Data Fig. 7: K-means clustering analysis of Type I DGC (DGC I), Type II DGC (DGC II), CA3 PCs, and CA1 PCs.
a-d, Mean centroids determined by k-means clustering into two clusters for CA3 PCs (a), DGC I (b), DGC II (c) and CA1 PCs (d). Dashed lines indicate 99%CI determined from bootstrapping iterations. e-h, Consistency analysis for with k = 2 clusters for CA3 PCs (e), DGC I (f), DGC II (g) and CA1 PCs (h). For each, heat map shows individual cell classification across each iteration. Upper Right) panel displays the probability of each iteration matching the classification of the full dataset. Bottom Left) panel displays the probability of a cell maintaining is classification identity across iterations. Lower Right) mean probabilities across iterations. Dashed lines indicate chance probability (50%). i-l, Consistency analysis for with k = 3 clusters for CA3 PCs (i), DGC I (j), DGC II (k) and CA1 PCs (l). m-p, Scatter plots indicating the comparative distance from centroid for each cell binned by age and genotype for CA3 PCs (m), DGC I (n), DGC II (o) and CA1 PCs (p). Unity line represents the decision line between each hyperexcitable and normal. q. Mean centroids from cluster analysis of combined DGCs. r) Violin plots of the residual values for combined DGCs across ages, genotype, and heuristically determined DGC types. For all panels: CA3 pyramidal cells: Young E3-KI: eight mice, n = 37 cells. Young E4-KI: seven mice, n = 25 cells. Aged E3-KI: six mice, n = 24 cells. Aged E4-KI: four mice, n = 21 cells. fE4-KISyn1-Cre+: three mice, n = 19 cells. fE4-KISyn1-Cre-: three mice, n = 19 cells. Dentate granule cells: Young E3-KI: four mice, n = 11 Type I DGCs, 22 Type II DGCs. Young E4-KI: three mice, n = 11 Type I DGCs, 21 Type II DGCs. Aged E3-KI: five mice, n = 24 Type I DGCs, 24 Type II DGCs. Aged E4-KI: five mice, n = 23 Type I DGCs, 20 Type II DGCs. CA1 pyramidal cells: Young E3-KI: four mice, n = 27 cells. Young E4-KI: four mice, n = 30 cells. Aged E3-KI: four mice, n = 30 cells. Aged E4-KI: four mice, n = 21 cells. Significance was assessed two-way ANOVA with Tukey’s post hoc multiple comparisons test.
