Fig. 7: snRNA-seq analysis of E3-KI and E4-KI mouse hippocampi at different ages. | Nature Aging

Fig. 7: snRNA-seq analysis of E3-KI and E4-KI mouse hippocampi at different ages.

From: Neuronal APOE4-induced early hippocampal network hyperexcitability in Alzheimer’s disease pathogenesis

Fig. 7: snRNA-seq analysis of E3-KI and E4-KI mouse hippocampi at different ages.

a, Experimental design: hippocampi were extracted from female E3-KI and E4-KI mice at 5 months, 10 months, 15 months and 20 months of age, and from female fE4-KI/Syn-Cre+ mice at 5 months and 10 months of age (n = 4 mice per genotype and age). The hippocampi were dissociated, and nuclei were labeled with DAPI and isolated using flow cytometry before processing using the 10x Chromium v2 system for snRNA-seq. b, Clustering using the Seurat package revealed 27 distinct cellular populations in E3-KI and E4-KI mice. Marker gene analysis led to the identification of 16 neuronal clusters (clusters 1–16) and 11 non-neuronal clusters (clusters 17–27). c, Candidate gene selection criteria for clusters 1, 2 and 6. Values represent the number of genes passing each successive criterion. See also Supplementary Fig. 1 and Supplementary Tables 3–7. OPC, oligodendrocyte progenitor cell; PV, parvalbumin; RELN, reelin; SST, somatostatin; VIP, vasoactive intestinal peptide. Icons in c created in BioRender; Zilberter, M. https://BioRender.com/e05iqcb (2025).

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