Extended Data Fig. 1: Age-dependent spatial learning and search strategy analysis in E4-KI and E3-KI mice.
a, Aged E4-KI mice (14-18 months) show significant variability in escape latencies compared to E3-KI mice, reflecting heterogeneity in spatial learning performance. b, Average escape latency variance across 5 days of hidden platform trials for each mouse. c, Schematic representation of the six distinct search strategies used by mice in the MWM test. d, Strategy use as % total for each hidden platform trial across 5 days for aged E3-KI (left) and E4-KI (right) mice. e, Average search strategy scores for day 2 of the hidden platform trials. f, Early CA3 IIS rates correlate significantly with strategy scores on day 2 of hidden trials in aged E4-KI mice. g, Relationships between DG IIS rates in young E-KI mice and 2 of hidden trials strategy scores at old age. h, Early CA1 IIS rates do not predict strategy scores on hidden day 2 in aged E-KI mice. All data in (b, e) are represented as mean ± s.e.m. For IIS analysis, n = 14 and 15 young E3-KI and E4-KI mice; n = 13 and 16 for aged E3-KI and E4-KI mice. MWM test, n = 9 and 12 for E3-KI and E4-KI mice. Significance was assessed using unpaired two-tailed Student’s t-test or Pearson’s correlation analysis (two-sided). Panel (c) created in BioRender https://BioRender.com/6sz2fev.
