Extended Data Fig. 2: The effects of age on BMDM-derived senescent macrophages.

(a) Senescence-associated β-galactosidase assay images of young and old BMDM senescent macrophages at 20x magnification. (b) Click-it EdU labeling of cells to assess the percentage of cells in S-phase. Bar graph quantification is an average of three independent biological replicates. (c) Mean, +/− SE, gene expression (RT-qPCR) normalized to young control. N = 3 biological replicates, p-value from Tukey-test post-ANOVA analysis. P.Control means passage control.