Extended Data Fig. 4: Evaluation of senescence in aging liver cells and aging heart cells.
(a) Mean, and +/− SE, gene expression (RT-qPCR) of Cdkn2a from N = 5, 2–4 month young (Y) vs. 21–24-month-old (O) mice. Asterisks represent the p-value of t-test ≥0.05 (ns), <0.05 (✱), <0.01 (✱✱), <0.001 (✱✱✱), <0.0001 (✱✱✱✱). (b) The percent cell is positive for Cdkn1a (blue) and Cdkn2a (red) across the remainder of liver cells across age. (c) Bubble map of genes grouped by hallmarks or macrophage senescence. Size represents the fraction of cells expressing each gene, and color reflects average expression for the remainder of liver cells. (d) UMAP analysis of the Tabula Muris Senis data set representing all cells in the liver annotated by cell type. UMAP analysis of the Tabula Muris Senis data set representing all cells in the liver annotated by age. (e) The percent cell is positive for Cdkn1a (blue) and Cdkn2a (red) across all cells in the heart and aorta data set.
