Extended Data Fig. 6: The effects of Trem2 −/− in senescent macrophages.
(a) Senescence-associated β-galactosidase assay imaging for wild-type and Trem2 −/− senescent macrophages at 20x magnification. Scale bars represent 20-micron distances. (b) Click-it EdU labeling of cells to assess % cells in S-phase. Bar graph quantification is an average of three independent biological replicates. N = 3 biological replicates, p-value from Tukey-test post-ANOVA analysis. (c) Gene ontology analysis for statistically significant upregulated DEGs within senescence in response to the Trem2 −/− background. The size of the data points represents the relative odds ratio.
