Extended Data Fig. 9: Effects of ABT-263 on gene expression, blood tests, liver enzymes, metabolites, and lipids. | Nature Aging

Extended Data Fig. 9: Effects of ABT-263 on gene expression, blood tests, liver enzymes, metabolites, and lipids.

From: p21+TREM2+ senescent macrophages fuel inflammaging and metabolic dysfunction-associated steatotic liver disease

Extended Data Fig. 9: Effects of ABT-263 on gene expression, blood tests, liver enzymes, metabolites, and lipids.The alternative text for this image may have been generated using AI.

(a) Relative Cdkn2a mRNA transcript levels of BMDMs from CETP-APOE* Leiden mice crossed on different three backgrounds. Asterisks represent the p-value of ANOVA ≥ 0.05 (ns), <0.05 (✱), <0.01 (✱✱), <0.001 (✱✱✱), <0.0001 (✱✱✱✱). (b-d) Serum metabolite, serum lipid levels, and serum liver enzymes related to pathology MASH mice. ND was observed outside blood glucose. Asterisks represent the p-value of t-test ≥0.05 (ns), <0.05 (✱), <0.01 (✱✱), <0.001 (✱✱✱), <0.0001 (✱✱✱✱). (e) Relative mRNA transcript levels for inflammatory genes in bulk visceral adipose (VAT) and kidney samples. P-value represents the results of the t-test. (f) Bulk-RNAseq of the M1 and M2 genes in response to the senolytic drug, ABT-263. Scale bar represents Log2[TPM + 1] expression on a z-score axis. N = 6 Vehicle, and N = 5 ABT-263 treated mice used in this experiment. (g) Pathology graded histology reports for fibrosis on 5-6 MASH mice with and without ABT-263. (h) Fold change differences of normalized lipid abundance (nmol lipid per mg liver) for all lipid species via shotgun lipidomics (LC-MS/MS) relative to vehicle. Asterisks represent the p-value of t-test ≥0.05 (ns), <0.05 (✱), <0.01 (✱✱), <0.001 (✱✱✱), <0.0001 (✱✱✱✱).

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