Extended Data Fig. 2: Representative threshold calls for Ki67 and luminal markers and IHC validation of IMC data. | Nature Aging

Extended Data Fig. 2: Representative threshold calls for Ki67 and luminal markers and IHC validation of IMC data.

From: Single-cell spatial atlas of the aging human breast

Extended Data Fig. 2: Representative threshold calls for Ki67 and luminal markers and IHC validation of IMC data.

a, Ki67, FOXA1, GATA3, ER, PR, AR, and HER2-positive cells depicted with white cell borders. Representative images are shown from patients selected from the cohort; similar staining patterns were observed across all patients included in the study. Scale bars for full images, 150 µm; scale bars for zoomed insets and HER2 images, 50 µm. b, Relationship between positive cell percentages as assessed by IHC and corresponding IMC normalised mean intensity for 6 markers. Correlation coefficients (ρ) and p-values are derived from two-sided Spearman rank correlation coefficient tests; loess regression lines are shown. IHC, immunohistochemistry; IMC, imaging mass cytometry.

Back to article page