Extended Data Fig. 3: Higher p21+ levels in tumors correlate with circulating MMP-7 levels and treatment-induced changes in serum MMP-7.
a, Plot of p21+ cells in the tumor versus MMP-7 level in the serum for vehicle, cisplatin, and pemetrexed-treated mice (n = 5 independent mice for vehicle / cisplatin group, and n = 6 independent mice for pemetrexed group, one-tailed Pearson correlation). Solid lines indicate linear regression fits (least-squares fit; estimated mean relationship between variables), and dashed lines denote the 95% confidence intervals of the regression line. b. Serum concentration of MMP-7, before and after treatment (n = 5 independent mice for vehicle / cisplatin group, and n = 6 independent mice for pemetrexed group; paired two-tailed t-test). c. Fold change of serum concentration of MMP-7 before and after treatment (n = 5 independent mice for vehicle / cisplatin group, and n = 6 independent mice for pemetrexed group, mean ± s.d., ordinary one-way ANOVA with Dunnett’s multiple comparisons). d. Correlation plot of MMP-7 in the serum versus tumor volume (n = 5 independent mice for vehicle / cisplatin group, and n = 6 independent mice for pemetrexed group, two-tailed Pearson correlation). Solid lines indicate linear regression fits (least-squares fit; estimated mean relationship between variables), and dashed lines denote the 95% confidence intervals of the regression line. e. Concentration of MMP-7 in the serum, before and after treatment, normalized to tumor volume before and after treatment (n = 5 independent mice for vehicle / cisplatin group, and n = 6 independent mice for pemetrexed group; paired two-tailed t-test). f. Fold change of concentration of MMP-7 in the serum before and after treatment normalized to tumor volume (n = 5 independent mice for vehicle / cisplatin group, and n = 6 independence mice for pemetrexed group; mean ± s.d., ordinary one-way ANOVA with Dunnett’s test multiple comparisons).
